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US 11,649,480 B2Granted
Method for modifying a template double stranded polynucleotide
AssigneeOxford Nanopore Technologies PLC
InventorsDavid Jackson Stoddart, James White
Life of this patent
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Abstract
The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.
Claims20
A method for modifying a template double stranded polynucleotide, comprising:
- (a) contacting the template polynucleotide with a MuA transposase and a population of double stranded MuA substrates each comprising an overhang at one or both ends of one strand such that the transposase fragments the template polynucleotide and ligates a substrate to one or both ends of the double stranded fragments and thereby producing a plurality of fragment/substrate constructs;
- and
- (b) using a translocase to remove the MuA transposases from the constructs and thereby producing a plurality of modified double stranded polynucleotides.
Dependencies are inferred from the claim text.