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US 11,649,473 B2Granted
Enzymatic modification of oil
AssigneeDSM Nutritional Products AG
InventorsJaroslav A. Kralovec, Weijie Wang, James Colin Barrow
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Abstract
The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.
Claims16
A method for modifying an oil comprising a glyceride, comprising:
- a) contacting an unmodified oil with a buffer solution of pH 10 and with water;
- b) contacting the unmodified oil with an aqueous solution of Thermomyces lanuginosus lipase at a time and temperature sufficient to hydrolyze at least a portion of the glyceride and provide a composition comprising a saturated free fatty acid fraction and a hydrolyzed glyceride fraction;
- c) separating at least a portion of the saturated free fatty acid fraction from the hydrolyzed glyceride fraction;
- and
- d) esterifying, in the presence of a second lipase, the hydrolyzed glyceride fraction from step c) with a polyunsaturated fatty acid or ethyl ester thereof to produce a modified oil,
- wherein the modified oil comprises greater than 40% omega-3 polyunsaturated fatty acids, 18% to 45% saturated fatty acids, 1% to 15% C18:1 fatty acids, 0% to 10% C16:1 fatty acids, and 60% to 80% of triglycerides, based on the total weight of the modified oil.
3 more elements
Dependencies are inferred from the claim text.