Wheat with increased resistant starch levels
AssigneeArcadia Biosciences, Inc.
InventorsAnn J. Slade, Dayna L. Loeffler, Aaron M. Holm, Jessica C. Mullenberg
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Abstract
A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.
Claims9
A process for producing a milled product, comprising:
- (i) providing wheat grain (Triticum durum) comprising an embryo and starch, wherein the embryo comprises two starch branching enzyme IIa (SBEIIa) genes, wherein both alleles of the SBEIIa-A gene are identical and both alleles of the SBEIIa-B gene are identical, and wherein
- (a) the SBEIIa-A gene has a severe mutation that is a short deletion and the SBEIIa-B gene has a severe mutation that is a short deletion;
- (b) SBEIIa enzyme activity is reduced or eliminated in the wheat grain;
- (c) the wheat grain has an increased amylose content compared to the amylose content of wild type wheat grain;
- and
- (d) the wheat grain is capable of germinating, and
- (ii) milling the grain to produce a milled grain product.
Dependencies are inferred from the claim text.