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US 11,649,264 B2Granted
Methods and products for genetic engineering
AssigneesInstitut National De La Sante Et De La Recherche Medicale (Inserm), Centre National de la Recherche Scientifique (CNRS), Ecole Normale Superieure De Lyon, Universite De Claude Bernard Lyon 1
InventorsThéophile Ohlmann, Philippe Mangeot, Emiliano Ricci
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Abstract
The present invention relates to a virus-derived particle comprising one or more Cas protein(s), as well as to kits and methods using the same for altering a target nucleic acid.
Claims48
A method of producing a retrovirus-derived particle comprising a Cas protein, the method comprising:
- a) providing a cell that comprises a nucleic acid sequence encoding a fusion protein comprising (i) a GAG protein and (ii) a Cas protein;
- b) culturing the cell under conditions that allow for production of a retrovirus-derived particle;
- and
- c) separating the retrovirus-derived particle from the cell, wherein the retrovirus-derived particle comprises the Cas protein contained within said particle.
1 more element
A method of producing a retrovirus-derived particle comprising a Cas protein, the method comprising:
- a) providing a cell that comprises a nucleic acid sequence encoding a GAG-Cas9 fusion protein;
- b) culturing the cell under conditions that allow for production of a retrovirus-derived particle;
- and
- c) separating the retrovirus-derived particle from the cell, wherein the retrovirus-derived particle comprises the Cas9 protein contained within said particle.
1 more element
Dependencies are inferred from the claim text.