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US 11,648,297 B2Granted
Bioresponsive particles
AssigneeBoard of Regents, The University of Texas System
InventorsJacques Lux, Robert F. Mattrey, Annie Y. Heble
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Abstract
Shielding enzymes are made by modifying the enzyme surface with silica precursors and then depositing silica to a desired thickness while retaining biological activity of the enzyme.
Claims20
A method of making hybrid enzyme-silica nanoparticles (HES-NPs), comprising the steps of:
- a) reacting acryloyl groups of acrylic compounds with amine groups of enzymes to covalently decorate the enzymes with enone groups;
- b) coupling silyl amines to the enone groups to covalently decorate the enzymes with silyl groups, forming silica-modified enzymes;
- c) growing siloxane scaffolds around the silica-modified enzymes, wherein the silyl groups seed the growth of the siloxane scaffolds to form the hybrid enzyme-silica nanoparticles, with each of the nanoparticles comprising a nanoporous silica shell encapsulating a plurality of the silica-modified enzymes;
- and
- d) isolating the hybrid enzyme-silica nanoparticles.
2 more elements
Dependencies are inferred from the claim text.