Human kynureninase enzymes and uses thereof
AssigneeBoard of Regents, The University of Texas System
InventorsGeorge Georgiou, Everett Stone, John Blazeck, Christos Karamitros
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Abstract
Methods and compositions related to the use of a protein with kynureninase activity are described. For example, in certain aspects there may be disclosed a modified kynureninase capable of degrading kynurenine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with kynurenine depletion using the disclosed proteins or nucleic acids.
Claims20
An isolated, modified kynureninase enzyme having at least 90% sequence identity to a human kynureninase enzyme comprising the amino acid sequence of SEQ ID NO:
- 1 and comprising at least one substitution selected from the group consisting of A99R, E103R, V104H, R107P, G112Y, I183P, and I331S, wherein the isolated, modified kynureninase enzyme has increased catalytic activity (kcat/kM) towards kynurenine as compared to the human kynureninase enzyme comprising the amino acid sequence of SEQ ID NO:
- 1.
An isolated, modified human kynureninase enzyme comprising the amino acid sequence of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.