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AssigneesAsc Therapeutics Inc., The Regents of the University of Michigan
InventorsRuby Yanru Tsai, Jie Xu, Yuqing Chen, Jifeng Zhang, Ling-Jie Kong
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Provided is a genetically modified non-human mammal that comprises an anchor DNA sequence inserted at an endogenous locus of a secretory milk protein gene, wherein the anchor DNA sequence comprises a site-specific recombinase recognition site. Also provided is a genetically modified non-human mammal that comprises a transgene inserted at an endogenous locus of a secretory milk protein gene, wherein the transgene encodes a secretory protein and is operably linked to the endogenous promoter of said secretory milk protein gene, and wherein the transgene is flanked by a pair of site-specific recombinase resulting sites. The genetically modified non-human mammals provided can be used for producing the secreted recombinant protein encoded by the transgene from the milk produced by the genetically modified non-human mammals.
A method for making a genetically modified rabbit, the method comprising:
The method of claim of 4, wherein the nucleic acid sequence encoding the exogenous protein comprises at its 3′ end a polyA sequence.
The method of claim of 4, wherein the exogenous protein is selected from the group consisting of antigen binding proteins, antibodies, vaccines, fusion proteins, enzymes, co-enzymes, clotting factors, and lysosomal proteins.
The method of claim of 4, wherein the exogenous protein is human factor VII (FVII) or peptide LL37.
Dependencies are inferred from the claim text.