Method of preparing polyvinyl alcohol nanofiber membrane enhancing cell specific adhesion
AssigneeNanofaentech Co., Ltd.
InventorsJong-Young Kwak, Minho Choi, Gwang Lee
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Abstract
A method of preparing a polyvinyl alcohol nanofiber membrane includes a material for controlling cell specific adhesion, and a nanofiber membrane that can maintain cellular functions such as cell activity and growth is prepared by adding aqueous solutions containing a polyacrylic acid and a glutaraldehyde crosslinking agent in a polyvinyl alcohol and materials capable of enhancing or regulating cell adhesion, electrospinning, treating with hydrochloric acid vapor and dimethylformaldehyde solvent and treating with sodium hydroxide to control the cell adhesion.
Claims6
A method of preparing polyvinyl alcohol nanofiber membrane having enhanced cell adhesion, comprising:
- (1) preparing polyvinyl alcohol nanofiber membrane by adding cell-adhesive material to an electrospinning solution containing polyvinyl alcohol (PVA), polyacrylic acid (PAA) and glutaraldehyde (GA) and electrospinning;
- (2) cross-linking the polyvinyl alcohol nanofiber membrane by hydrochloric acid (HCl) vapor treatment, followed by crystallization by treating with dimethylformamide (DMF) solvent;
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- (3) treating crystallized polyvinyl alcohol nanofiber membrane with sodium hydroxide.
Dependencies are inferred from the claim text.