Portable fluidic platform for rapid cell-free production of protein biologics

A cell-free method for producing an amount of at least one protein biologic formulation at pharmaceutical dosage levels at a point of need, the method comprising:

• loading by a loading module a reaction solution including one or more reaction reagents to a reactor module;
• loading by a feeder module a feeding solution to the reactor module, the reactor module having a first chamber and a second chamber divided by a membrane;
• receiving at the first chamber the one or more reaction agents from the loading module;
• receiving at the second chamber the feeding solution from the feeder module;
• producing by the reactor module a target protein via a process selected from the group consisting of:
• (i) continuous exchange cell-free (CECF) and (ii) batch protein synthesis;
• monitoring the produced target protein by one or more analytical modules to asses target protein purity level;
• directing the produced target protein to one of multiple separation modules in accordance with target protein requirements;
• separating the target protein from one or more components of the reaction solution;
• and
• formulating by a finishing module the target protein;
• wherein the producing, monitoring, directing, separating and formulating of the target protein are completed within a period of 24 hours.