Macrobrachium nipponense Cathepsin L gene, dsRNA thereof, and use thereof

A method of preparing an inhibitor, wherein the inhibitor inhibits ovary development of Macrobrachium nipponense, which comprises:

• providing a first and a second pair of interference primers that hybridize to a Macrobrachium nipponense Cathepsin L gene,
• collecting total cDNA of Macrobrachium nipponense as a template,
• performing PCR with the total cDNA in the presence of the first and second pair of interference primers to obtain a first and a second interference gene fragment, respectively, and
• performing RNA synthesis with the first and second interference gene fragments as templates to generate a first dsRNA and a second dsRNA;
• wherein the first dsRNA and the second dsRNA are both individually inhibitors of ovary development in Macrobrachium nipponense;
• wherein the nucleotide sequence of the Macrobrachium nipponense Cathepsin L gene is set forth in SEQ ID NO. 1;
• and
• wherein the amino acid sequence of the protein encoded by the Macrobrachium nipponense Cathepsin L gene is set forth in SEQ ID NO. 2;
• wherein the nucleotide sequences of the first and the second interference gene fragments are SEQ ID NO:3 and SEQ ID NO:8, respectively.