Device and method for producing and purifying exosomes

A device for producing and purifying exosomes from progenitor cells, wherein the device comprises a closed, sterile circuit having a pumping circuit and a plurality of serially connected processing stations, wherein

• a first processing station comprises a first culture medium containing said progenitor cells, which are cultured for the production of said exosomes;
• a second processing station comprises a second medium conditioned by the exosomes produced in the first processing station;
• a third processing station comprises a filter clarifying said conditioned medium;
• a fourth processing station comprises one or more hollow fiber cartridges concentrating the clarified conditioned medium by tangential filtration and a recirculator recirculating the concentrated medium for a subsequent concentration thereof;
• a fifth processing station comprises a formulation buffer diluting the concentrated medium;
• a sixth processing station comprises waste material output from said fourth processing station;
• a seventh processing station comprises a collector collecting the diafiltered-concentrated medium containing the purified exosomes;
• and
• an eighth processing station comprises a storage storing said purified exosomes.