Display substrate, manufacturing method thereof, and display panel

A display substrate, comprising:

• a base substrate;
• a plurality of sub-pixels in an array on the base substrate, wherein adjacent sub-pixels are separated by a pixel defining layer, each of the plurality of sub-pixels includes a first electrode, a light-emitting layer and a second electrode in a direction away from the base substrate, the second electrode of each of the plurality of sub-pixels is connected to one another to form a second electrode layer;
• an isolation layer on a side of the second electrode layer opposite from the light-emitting layer;
• and
• an auxiliary conductive layer directly disposed on a side of the isolation layer opposite from the second electrode layer,
• wherein the isolation layer comprises a via opening, orthographic projection of the via opening on the base substrate substantially overlaps with orthographic projection of the pixel defining layer on the base substrate, orthographic projection of the auxiliary conductive layer on the base substrate covers the orthographic projection of the pixel defining layer and orthographic projection of the light-emitting layer on the substrate, and the auxiliary conductive layer is connected to the second electrode layer through the via opening;
• the display substrate further includes a plurality of pixels arranged along a first direction with a pixel interval, each of the plurality of pixels includes a set of adjacent sub-pixels arranged along a first direction with a sub-pixel interval, the set of adjacent sub-pixels includes more than one sub-pixel, and the sub-pixel interval is shorter than the pixel interval, the via opening is located between two adjacent pixels but not between two adjacent sub-pixels of a same pixel and surrounds each of the plurality of pixels, and the set of adjacent sub-pixels and sub-pixel intervals between the adjacent sub-pixels are covered by the isolation layer.