Systems and methods for imaging a sample
AssigneePurdue Research Foundation
InventorsGarth Jason Simpson, James Ulcickas, Fengyuan Deng, Changqin Ding
Life of this patent
Estimating the term…
Abstract
A method for imaging a sample, wherein the sample changes a polarization state of light as a function of position, wherein the method includes changing a polarization state of a purely polarized light of an incident light striking a micro-retarder array, thereby inducing a changed polarization state of the polarization state. The micro-retarder array is placed in a rear conjugate focal plane of a microscope. The method additionally includes projecting the changed polarization state of the polarization state into an object plane of the microscope containing the sample.
Claims10
A method for imaging a sample, wherein the method comprises:
- changing a polarization state of a purely polarized light of an incident light striking a micro retarder micro-retarder array, thereby inducing a changed polarization state of the polarization state, wherein the polarization state is rotated as a function of a translation across the micro-retarder array to form the changed polarization state, wherein the micro-retarder array is placed in a rear conjugate focal plane of a microscope;
- projecting the changed polarization state of the polarization state into an object plane of the microscope containing the sample;
- translating the sample through a pattern of polarizations induced by the micro-retarder array to interrogate each location of the sample with multiple polarizations;
- performing second harmonic generation of the purely polarized light;
- and
- imaging the sample.
A method for imaging a sample, wherein the method comprises:
- directing a purely polarized light toward a micro-retarder array;
- changing a polarization state of the purely polarized light striking the micro-retarder array, thereby inducing a changed polarization state of the polarization state, wherein the polarization state is rotated as a function of the translation of the purely polarized light across the micro-retarder array;
- projecting the changed polarization state of the polarization state into an object plane of the microscope containing the sample;
- translating the sample through a pattern of polarizations induced by the micro-retarder array to interrogate each location of the sample with multiple polarizations;
- determining a Stokes and Mueller set of parameters, wherein the Stokes and Mueller set of parameters dictate a linear polarization transfer properties of the sample;
- and
- imaging the sample.
A method for imaging a sample, wherein the method comprises:
- changing a polarization state of a purely polarized light of an incident light striking a micro-retarder array, thereby inducing a changed polarization state of the polarization state, wherein the micro-retarder array includes a linearly varying principal azimuthal rotation of polarization defined along a single spatial axis in the micro-retarder array, and wherein the micro-retarder array is placed in a rear conjugate focal plane of a microscope;
- projecting the changed polarization state of the polarization state into an object plane of the microscope containing the sample;
- combining image reconstruction algorithms with a single image, thereby producing a combined polarization dependent image, wherein the single image comprises a low frequency power spectrum, wherein the single image is an image of the sample;
- deconstructing the combined polarization dependent image to produce a plurality of sparsely sampled purely polarized images;
- inpainting, using software, the plurality of sparsely sampled purely polarized images, thereby recovering a set of polarization dependent images;
- and
- imaging the sample.
Dependencies are inferred from the claim text.