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AssigneeLeica Microsystems CMS GmbH
InventorsAlexander Weiss, Christian Schumann, Ronja Capellmann
Estimating the term…
A method is useable for determining a refractive index of an optical medium in a microscope, which has an objective facing toward a sample chamber. The optical medium is one of two optical media, which border two opposing surfaces of a cover slip or object carrier in the sample chamber and form two partially reflective interfaces, which are arranged at different distances from the objective. The method includes: deflecting a measurement light beam by the objective with oblique incidence on the cover slip or object carrier; generating two reflection light beams spatially separated from one another by the measurement light beam being partially reflected at each of the interfaces; receiving the two reflection light beams by the objective and conducting them onto a position-sensitive detector; registering intensities by the position-sensitive detector; and determining the refractive index of the optical medium based on the registered intensities.
A method for determining a refractive index of an optical medium in a microscope, which has an objective facing toward a sample chamber, wherein the optical medium having the refractive index to be determined is one of two optical media, which border two opposing surfaces of a cover slip or object carrier in the sample chamber and form two partially reflective interfaces, which are arranged at different distances from the objective, the method comprising:
A microscope, comprising:
Dependencies are inferred from the claim text.