US 11,635,607 B2 · Spectroscopic single-molecule localization microscopy

Abstract

A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order (“1st”) light and a negative first order (“−1st”) light using a grating; wherein the 1st light comprises spectral information and the −1st light comprises spectral information; capturing the 1st light and the −1st light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (“DWP”) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.

Claims20

Dependent claims

A spectrometer for a microscope, the spectrometer comprising:

a monolithic optic, the monolithic optic comprising:
a beam splitter configured to split an incident light into a first beam and a second beam, wherein the first beam forms a spatial image at a first image plane, the first beam and the spatial image comprising spatial information;
and
a dual-wedge prism (“DWP”) having a first dispersive optical device and a second dispersive optical device, the DWP being configured to receive the second beam, to spectrally disperse the second beam into a third beam, and to direct the third beam to a second image plane, wherein the second beam interacts with the first dispersive optical device and wherein the third beam exits the second dispersive optical device and forms a spectral image at the second image plane, the third beam and the spectral image comprising spectral information,
wherein the first image plane and the second image plane are parallel, and
wherein the first image plane and the second image plane are each independently perpendicular to an optical axis.

3 more elements

Dependencies are inferred from the claim text.

Spectroscopic single-molecule localization microscopy

Life of this patent

Spectroscopic single-molecule localization microscopy

Life of this patent

Abstract

Claims20