Microscope apparatus and program

A microscope apparatus comprising:

• a processor;
• a focusing device capable of forming an image of observation target light indicating an observation target in a container in which the observation target is contained, on an imaging element, including an objective lens, a phase plate and an imaging lens;
• a drive source that includes a first moving member movable for moving the objective lens along an optical axis of the focusing device, and a second moving member movable along the optical axis in a range wider than that of the first moving member and moves the objective lens along the optical axis using the first moving member and the second moving member;
• and
• a displacement sensor that is provided along the optical axis and detects a position of an observation scheduled region along the optical axis and is positioned forward in a scanning direction relative to the optical axis of the focusing device and imaging element,
• wherein the processor configured to
• control the drive source to cause the imaging element to form the image of the observation target light in a focus state when the optical axis of the focusing device and imaging element reaches the observation scheduled region, by moving the focusing device along the optical axis by using the first moving member and the second moving member based on information on a distance along the optical axis between the observation scheduled region and a region to be imaged by the imaging element at an imaging position, in a case where it is determined that the observation scheduled region exists at a position of an out-of-focus state in a state where the first moving member is moved to a limit of a movable range of the first moving member before the optical axis reaches the observation scheduled region among respective regions, in a state where the imaging element scans the respective regions in the container by moving the focusing device with respect to the respective regions in the container by a movement of at least one of a stage on which the container is installed or the focusing device in a plane intersecting the optical axis;
• and
• control the drive source to cause the imaging element to form the image of the observation target light in the focus state when the optical axis reaches the observation scheduled region by applying power along the optical axis by the first moving member and the second moving member to the focusing device based on the information on the distance, in a case where a position detected by the displacement sensor is the position of the out-of-focus state in a state where the first moving member is moved to the limit of the movable range, before the optical axis reaches the observation scheduled region among the respective regions in a state where the imaging element scans the respective regions in the container.