Sandwich assays in droplets
AssigneeBio-Rad Laboratories, Inc.
InventorsMichael L. Samuels, Darren Roy Link
Life of this patent
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Abstract
The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.
Claims20
An analysis method comprising:
- encapsulating cells into a plurality of droplets to create at least one droplet containing a single cell, wherein the droplet includes one or more binding agents, wherein the binding agents provide an analyte identifier for a target analyte and an identifier specific for the droplet;
- lysing the cell within the droplet to release the target analyte;
- binding the one or more binding agents to the target analyte;
- and
- detecting the target analyte identifier, thereby detecting the target analyte.
A droplet library comprising:
- a plurality of droplets, wherein at least one droplet comprises, in an aqueous phase fluid,
- a single cell,
- one or more binding agents that provide an analyte identifier for a target analyte and an identifier specific for the droplet, and
- lysis reagents to lyse the cell.
Dependencies are inferred from the claim text.