Test strip and kit for testing mycophenolic acid and preparation method of test strip

A test strip for testing the content of mycophenolic acid, comprising a bottom plate and a sample pad, a glassfiber membrane, a nitrocellulose membrane and an absorbent paper which are successively lapped on a surface of the bottom plate, wherein

• the sample pad is treated by a sample pad treatment fluid, and the sample pad treatment fluid comprises a buffer solution, an active protein and a surfactant, wherein the active protein is bovine serum albumin;
• the glassfiber membrane is treated by a glassfiber membrane treatment fluid, and the glassfiber membrane is coated by a mycophenolic acid specific-antibody conjugate;
• and the mass concentration of the mycophenolic acid specific-antibody conjugate is 0.1-0.3%, and the coating concentration of the mycophenolic acid specific-antibody conjugate is 0.5-1 mg/mL, wherein the glassfiber membrane treatment fluid comprises a basal solution and following components at concentrations:
• 0.5-5 g/L polyethylene glycol 6000, 3-15 g/L mannitol, 0.1-1.5 g/L alum and 0.8%-1.0% sodium chloride;
• and the basal solution is a TRIS buffer solution;
• and
• the nitrocellulose membrane is provided with a detection line and quality control line, a mycophenolic acid protein conjugate is sprayed on the detection line, the concentration of the mycophenolic acid protein conjugate is 1-3 mg/ml, and the spraying amount is 1-3 μL/cm.