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US 11,635,387 B2Granted
Optical imaging system using lateral illumination for digital assays
AssigneeAbbott Japan Co., Ltd.
InventorTomohiro Ikeda
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Abstract
A compact optical imaging system including a single filter and a light source that provides lateral illumination for bead detection in digital assays. The light source is configured to emit light toward the detection vessel. The single filter is positioned to receive light reflected from a sample in the detection vessel, that originated from the light source, and receive an output from a sample in the detection vessel. A detector is configured to receive a portion of the reflected light and a portion of the output that passes through the single filter.
Claims14
A method of detecting an analyte of interest in wells comprising:
- (a) contacting a sample suspected of containing an analyte with
- (i) a detection vessel comprising a plurality of wells with at least one bead within a well, wherein the at least one bead comprises a specific binding member that binds to the analyte of interest;
- (ii) a light source configured to emit light toward the detection vessel;
- (iii) a filter positioned to receive and pass through a portion of light reflected that originated from the light source from the at least one bead, and receive and pass through a portion of a fluorescence output from the at least one bead in the detection vessel;
- and (iv) a detector configured to receive a portion of the reflected light and a portion of the fluorescence output that passes through the single filter;
- and
- (b) determining which wells contain a bead using information from the reflected light and the fluorescence outputs.
4 more elements
Dependencies are inferred from the claim text.