Extracellular vesicle isolation by nanomembranes

A method for extracellular vesicle isolation comprising the steps of:

• (a) collecting a sample;
• (b) contacting the sample with a pre-filtration membrane under normal flow, wherein the pre-filtration membrane is a high permeability silicon nanomembrane and wherein the pre-filtration membrane is functionalized with non-fouling surface treatments by a silanization process, a carbinylation process or an epihalohydrin-based process, and forming a first filtrate and a first retentate;
• (c) washing the first retentate of the pre-filtration membrane under normal flow;
• (d) contacting the first filtrate from the pre-filtration membrane with a first capture membrane under normal flow, wherein the first capture membrane is a high permeability silicon nanomembrane and wherein the first capture membrane is functionalized with non-fouling surface treatments by a silanization process, a carbinylation process or an epihalohydrin-based process, and forming a second filtrate and a second retentate;
• and
• (e) washing the second retentate under normal flow.