US 11,635,354 B2 · Spatial molecular profiling of solid biological masses and profile storage

Abstract

Disclosed is a method for capturing the spatial molecular profiling of a biological mass formed from biological material, comprising the steps of: a) providing a transected biological mass, for example a tumour, the transection exposing at least a portion of the mass; b) providing a solid support of an area at least equalling the area of said portion of the mass; c) transferring biological material from the portion of the mass to the support to provide on the support a two dimensional imprint of the biological material present at the portion of the mass; and d) performing a biological assay of the transferred biological material from different predetermined locations of the imprint in order to determine the spatial molecular profile of the portion of the mass.

Claims8

Dependent claims

An apparatus for capturing a spatial profile of biological material from at least a portion of a biological mass, the apparatus comprising:

a biological mass tray configured to hold the biological mass and to hold a solid support;
chemicals;
and
the solid support, the solid support defining a support surface having a contact area configured to contact at least the portion of the mass to retain a two dimensional imprint of the portion of the biological mass, the solid support further including a preformed reference grid at least at said contact area,
wherein the support surface is impregnated with the chemicals, and
wherein the chemicals include a weak base, a chelating agent, an anionic surfactant, or a chaotropic agent.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/318,297Filed 23 Jun 2015

Priority

GB 1411624.830 Jun 2014

Published

US 2017/0131188 A111 May 2017

Granted

25 Apr 2023

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

G01N 1/2813
Preparing specimens for investigation including physical details of chemical methods covered elsewhere, e.g. G01N33/50, C12Q: producing thin…
B01L 3/508
Containers for the purpose of retaining a material to be analysed, e.g. test tubes: rigid containers without fluid transport within
C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6841
Hybridisation assays: in situ hybridisation

Spatial molecular profiling of solid biological masses and profile storage

Life of this patent

Spatial molecular profiling of solid biological masses and profile storage

Life of this patent

Abstract

Claims8