Method for diagnosis and subtyping of adult onset Still's disease

A method for detecting or diagnosing a subject suffering from Adult-onset Still's disease (AOSD), the method comprising the steps of:

• (a) providing a blood sample and a peripheral blood mononuclear cell from the subject;
• (b) detecting the expression level of a protein biomarker in the blood sample, wherein the protein biomarker is IL-18;
• detecting expression levels of a long non-coding ribonucleic acid (lncRNA) from the peripheral blood mononuclear cell, wherein the lncRNA comprises myocardial infarction associated transcript (MIAT) and TNFα and hnRNPL-related immunoregulatory lincRNA (THRIL);
• (c) calculating an A score of the detected IL18, MIAT and THRIL obtained in step (b) through a prediction equation, which is determined by performing a multiple regression analysis;
• (d) comparing the A score calculated in step (c) with a cutoff value, wherein the cutoff value is obtained by a Receiver Operator Characteristic (ROC) method, wherein the cutoff value corresponds to the area under an ROC curve (AUC) at its maximum, and the ROC method is established based on the expression of the lncRNA and IL-8 of AOSD patients and healthy subjects, wherein the A score being higher than the cutoff value indicates that the subject suffers from AOSD;
• and
• (e) administering an effective amount of AOSD-treating drugs to the subject.