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US 11,634,772 B2Granted
Antidote-mediated reversal of extracellular aptamer staining
AssigneeDuke University
InventorsBruce Sullenger, Bethany Gray, Michael Nichols
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Abstract
Provided herein are compositions and methods for sorting and/or identifying live cells. The compositions and methods provide for staining of live cells with aptamer so particular cells can be identified within or sorted from a heterogeneous population of live cells and subsequent reversal of the staining to prepare sorted and/or identified cells in their native state.
Claims15
A method for live cell identification, the method comprising:
- (a) providing a heterogeneous population of live cells, the heterogeneous population of live cells comprising a cell having a surface-bound molecular target;
- (b) contacting the surface-bound molecular target with an aptamer conjugate having a binding affinity for the molecular target;
- (c) identifying the cell having the surface-bound molecular target amongst the heterogeneous population of cells using the aptamer conjugate;
- and
- (d) removing the aptamer conjugate from the surface-bound molecular target to prepare a sorted cell in its native state,
- wherein the aptamer conjugate comprises a multiplicity of sorting aptamers each comprising a binding label complexed to a linker by binding of the binding label to the linker;
- and
- wherein the linker comprises a reporter label and/or a magnetic label.
5 more elements
A method for sorting a live cell in its native state from a heterogeneous population, the method comprising:
- (a) providing a heterogeneous population of live cells, the heterogeneous population of live cells comprising a cell having a surface-bound molecular target;
- (b) contacting the surface-bound molecular target with an aptamer conjugate having a binding affinity for the molecular target;
- (c) sorting the cell having the surface-bound molecular target from at least a portion of the heterogeneous population of live cells using the reporter label and/or a magnetic label;
- and
- (d) removing the aptamer conjugate from the surface-bound molecular target to prepare a sorted cell in its native state,
- wherein the aptamer conjugate comprises a multiplicity of sorting aptamers each comprising a binding label complexed to a linker by binding of the binding label to the linker;
- and
- wherein the linker comprises the reporter label and/or the magnetic label.
5 more elements
Dependencies are inferred from the claim text.