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US 11,634,763 B2Granted
Enzyme method
AssigneeOxford Nanopore Technologies PLC
InventorsRuth Moysey, Andrew John Heron
Life of this patent
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Abstract
The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.
Claims20
A method of characterising a target polynucleotide, comprising:
- (a) contacting the target polynucleotide with a transmembrane pore under an applied voltage field such that a portion of the target polynucleotide is captured by the transmembrane pore, wherein the pore is present in a membrane;
- (b) contacting the target polynucleotide with a Hel308 helicase on the cis side of the membrane such that the helicase binds to the target polynucleotide, thereby controlling the movement of the target polynucleotide through the transmembrane pore against the applied voltage field from the trans side of the membrane to the cis side of the membrane;
- and
- (c) measuring one or more characteristics of the target polynucleotide as the target polynucleotide moves through the transmembrane pore, thereby characterising the target polynucleotide.
1 more element
Dependencies are inferred from the claim text.