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US 11,634,760 B2Granted
Method for amplifying target nucleic acid and composition for amplifying target nucleic acid
AssigneePanagene Inc.
InventorsJaejin Park, Minseo Kim
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Abstract
The present invention relates to a method for detecting a target nucleic acid, which induces any surrogate target to be amplified in the presence of the target nucleic acid and is useful for molecular diagnosis, prenatal diagnosis, early diagnosis, cancer diagnosis, genetic related diagnosis, genetic trait diagnosis, diagnosis of infectious bacteria, identification of drug-resistant bacteria, forensic medicine, species identification of organisms, and the like.
Claims21
A method for amplifying a target nucleic acid, the method comprising steps of:
- (a) isolating a nucleic acid from a sample;
- (b) performing a polymerase chain reaction (PCR) by adding
- i) at least one target-specific primer capable of hybridizing with the target nucleic acid,
- ii) at least one surrogate target comprising a sequence that binds to a target nucleic acid region to which the target-specific primer does not bind, and an arbitrary sequence that does not bind to the target nucleic acid, and
- iii) at least one amplification primer capable of amplifying the surrogate target;
- and
- (c) determining the presence or absence of a fusion amplicon.
4 more elements
Dependencies are inferred from the claim text.