Crispr enzymes and systems
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Teohnology, President and Fellows of Harvard College
InventorsFeng Zhang, Bernd Zetsche, Johnathan S. Gootenberg, Omar O. Abudayyeh, Ian Slaymaker
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Abstract
The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA. Methods for making and using and uses of such systems, methods, and compositions and products from such methods and uses are also disclosed and claimed.
Claims30
A composition comprising, (a) a Cas polypeptide that comprises a RuvC-like nuclease domain and a zinc finger, but does not comprise an HNH domain, or a polynucleotide encoding the Cas polypeptide, and (b) one or more engineered nucleic acid components lacking a tracr sequence that form a CRISPR-Cas complex with the Cas polypeptide and that is capable of directing sequence-specific binding of said complex to a target polynucleotide sequence adjacent to a protospacer adjacent motif (PAM) in the genome of a eukaryotic cell, or a polynucleotide encoding the one or more engineered nucleic acid components.
A composition comprising (a) a Cas polypeptide that comprises a RuvC-like nuclease domain, but does not comprise an HNH domain, or a polynucleotide encoding the Cas polypeptide, and (b) one or more engineered nucleic acid components lacking a tracr sequence that form a complex with the Cas polypeptide and that is capable of directing sequence-specific binding of said complex to a target polynucleotide sequence adjacent to a protospacer adjacent motif (PAM) in the genome of a eukaryotic cell, or a polynucleotide encoding the one or more engineered nucleic acid components, wherein the Cas polypeptide comprises at least one mutation in the RuvC-like nuclease domain and is catalytically inactive.
Dependencies are inferred from the claim text.