On-slide staining by primer extension
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsNikolay Samusik, Garry P. Nolan, Yury Goltsev, David Robert McIlwain
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Abstract
A method for analyzing planar sample is provided. In some cases the method comprises: (a) labelling the planar sample with a capture agent that is linked to a nucleic acid, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the nucleic acid, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
Claims20
A method of analyzing a planar sample, the method comprising:
- obtaining a labeled composition comprising:
- (a) a biological sample;
- (b) a plurality of capture agents;
- (c) a labeled oligonucleotide;
- and
- (d) a ligase;
- wherein, (i) the plurality of capture agents are linked to double-stranded oligonucleotides comprising a first strand and a second strand, wherein the ligase is configured to add the labeled oligonucleotide to the first strand or the second strand, and (ii) the plurality of capture agents are chemically cross-linked directly to the biological sample;
- and
- reading a signal generated by the labeled composition.
Dependencies are inferred from the claim text.