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US 11,634,752 B2Granted
Kit for split-pool barcoding target molecules that are in or on cells or cell organelles
AssigneeRoche Sequencing Solutions, Inc.
InventorGarry P. Nolan
Life of this patent
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Abstract
The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
Claims26
A kit for split-pool barcoding target molecules that are in or on cells or cell organelles, comprising:
- (i) at least two sets of assayable polymer subunit (APS) oligonucleotides, wherein each set comprises at least 10 APS oligonucleotides that each comprise:
- a 5′ end sequence that is common to the APS oligonucleotides in the set,
- a 3′ end sequence that is common to the APS oligonucleotides in the set, and
- an internal sequence that distinguishes the APS oligonucleotides in the set from one another, and
- (ii) one or more linking oligonucleotides, wherein a linking oligonucleotide is complementary to the 5′ end sequence of the APS oligonucleotides of one set and the 3′ end sequence of the APS oligonucleotides of another set,
- wherein the APS oligonucleotides from different sets are configured to link together in an ordered fashion in the presence of the one or more linking oligonucleotides to form all or part of a cell or organelle origination barcode.
3 more elements
Dependencies are inferred from the claim text.