Systems and methods for sample preparation for enzymatic A1C detection and quantification

A system for preparing a sample containing hemoglobin HbA1c for measurement by an electrochemical sensor, the system comprising:

• a lysing mixture, the lysing mixture including a zwitterionic surfactant;
• an oxidizing mixture, the oxidizing mixture including a cationic surfactant and an isothiazoline derivative;
• and
• a protease mixture, the protease mixture including a molecule including an azole, wherein the system includes a sampler/mixer device, and the lysing mixture is located in the sampler/mixer device, wherein the sampler/mixer device is configured to dose a sample area of a test strip, wherein the zwitterionic surfactant is n-Tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (Zwittergent 3-14®) and the cationic surfactant is 1-Dodecyl pyridinium chloride and the isothiazoline derivative is 1,2 Benzisothiazol-3(2H)-one and at a 1:5 dilution.