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US 11,634,737 B2Granted
Oleic acid production in yeast
AssigneeGinkgo Bioworks, Inc.
InventorsVasiliki Tsakraklides, Elena E. Brevnova, Jonathan Friedlander, Annapurna Kamineni, Arthur J. Shaw, IV
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Abstract
Disclosed are transformed cells comprising one or more genetic modifications that affect the lipid content of the cell, e.g., by increasing the concentration of oleic acid in the cell relative to an unmodified cell of the same type. Also disclosed are methods for modifying the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the same cell.
Claims22
A yeast cell comprising:
- a first genetic modification, wherein the first genetic modification is a knockout mutation of a native Δ12 desaturase protein;
- a second genetic modification, wherein the second genetic modification increases the expression of an elongase protein, a diacylglycerol acyltransferase protein, or a glycerol-3-phosphate acyltransferase protein;
- and
- a third genetic modification, wherein the third genetic modification results from a transformation with a nucleic acid comprising a gene encoding an exogenous fungal Δ9 desaturase protein.
1 more element
Dependencies are inferred from the claim text.