SEC modified strains for improved secretion of recombinant proteins
AssigneeBolt Threads, Inc.
InventorThomas Stevens
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Abstract
Disclosed herein are modified strains for improving secretion of recombinantly expressed products secreted from a host organism with improved growth and productivity characteristics, as well as methods of using the modified strains.
Claims23
A Pichia pastoris microorganism, in which the activity of a SEC72 protein comprising a polypeptide sequence of SEQ ID NO:
- 2 has been eliminated, and wherein said microorganism expresses a recombinant silk-like protein comprising a secretion signal peptide derived from yeast and selected from a PEP4 signal sequence, a CPY+4 signal sequence, a DAP2 signal sequence, and a MFα1 signal sequence.
A method of modifying a Pichia pastoris microorganism to improve the secretion of a recombinantly expressed protein, sad method comprising knocking out a gene encoding an SEC72 protein, and wherein sad microorganism expresses a recombinant silk-like protein comprising a secretion signal peptide derived from yeast and selected from a PEP4 signal sequence, a CPY+4 signal sequence, a DAP2 signal sequence, and a MFa1 signal sequence.
Dependencies are inferred from the claim text.