Plant gene editing systems, methods, and compositions
AssigneeInari Agriculture Technology, Inc.
InventorsErik William Clement, Yajie Niu, Hannah Pham
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Abstract
Systems, methods, and compositions that provide for increased frequencies of plant gene editing in comparison to controls are provided herein. Also disclosed are compositions that provide for editing target genes in plant cells. Systems for modification of a plant genome and/or target plant gene are provided. Methods for modifying a plant cell genome or a target plant gene in a plant cell genome are also provided. Compositions, plant cell cultures, and/or reaction mixtures comprising plant cells, certain agents, and gene editing molecules are also provided herein.
Claims10
A method for increasing Homology Directed Repair (HDR)-mediated genome modification of a plant cell genome, comprising:
- providing genome editing molecules to a plant cell,
- wherein the plant cell is exposed for at least 2 hours to:
- (i) at least one of an exogenous, heterologous, and/or overproduced agent comprising a geminivirus RepA protein, a CYREN protein, or biologically active fragment thereof, or polynucleotide encoding said protein or biologically active fragment thereof, or any combination thereof;
- and
- (ii) a hypoxic condition;
- wherein the genome editing molecules comprise an RNA-guided nuclease or a polynucleotide encoding an RNA-guided nuclease, a guide RNA or a polynucleotide encoding a guide RNA, and a donor template polynucleotide or a polynucleotide encoding a donor template polynucleotide;
- and
- wherein a geminivirus Rep protein is absent
- whereby the genome editing molecules modify the plant cell genome by HDR at a frequency that is increased in comparison to a control.
Dependencies are inferred from the claim text.