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US 11,634,719 B2Granted
Curing for recursive nucleic acid-guided cell editing
AssigneeInscripta, Inc.
InventorsCharles Johnson, Tian Tian, Eileen Spindler
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Abstract
The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.
Claims26
A composition of matter for recursive nucleic acid-directed nuclease CRISPR editing comprising one or more vectors, wherein the one or more vectors comprise:
- (i) a DNA sequence encoding one or more editing gRNAs under the control of a first inducible promoter;
- (ii) one or more donor DNA sequences, wherein the one or more editing gRNAs and the one or more donor DNA sequences edit one or more target sequences in a cell;
- (iii) a curing target sequence, wherein the curing target sequence is capable of being cut to cleave the one or more vectors;
- (iv) one or more selectable markers;
- and
- (v) a DNA sequence encoding a curing gRNA sequence under the control of a second inducible promoter, wherein the curing gRNA sequence targets the curing target.
3 more elements
Dependencies are inferred from the claim text.