Methods for preparing analytes and related kits

A nucleic acid-analyte composition comprising a plurality of nucleic acid-analyte chimeras and a plurality of capture nucleic acids attached to a solid support, the nucleic acid-analyte composition generated by the steps of:

• attaching a polypeptide analyte to a bait nucleic acid to generate a nucleic acid-analyte chimera, wherein the bait nucleic acid is covalently attached to the polypeptide analyte to generate the nucleic acid-analyte chimera;
• bringing the nucleic acid-analyte chimera into proximity with the solid support by hybridizing the bait nucleic acid in the nucleic acid-analyte chimera to a capture nucleic acid attached to the solid support;
• covalently coupling the nucleic acid-analyte chimera to the solid support, wherein the plurality of nucleic acid-analyte chimeras is coupled on the solid support and adjacent nucleic acid-analyte chimeras coupled on the solid support are spaced apart at an average distance of about 50 nm or greater;
• contacting the polypeptide analyte covalently coupled to the solid support with a binding agent capable of binding to the polypeptide analyte, wherein the binding agent comprises a coding tag that comprises identifying information regarding the binding agent;
• and
• transferring the identifying information regarding the binding agent from the coding tag to the bait nucleic acid or capture nucleic acid attached to the polypeptide analyte.

A kit for polypeptide analysis, comprising:

• (a) a plurality of bait nucleic acids, each of the bait nucleic acids is configured to be covalently attached to a polypeptide analyte;
• (b) a solid support comprising a plurality of capture nucleic acids attached hereto, each of the capture nucleic acids comprising a sequence complementary to a corresponding bait nucleic acid of the plurality of bait nucleic acids and configured to covalently couple the polypeptide analyte to the solid support, wherein adjacent capture nucleic acids are spaced apart on the solid support at an average distance of about 50 nm or greater;
• and
• (c) one or more binding agents capable of binding to the polypeptide analyte, wherein each binding agent comprises a coding tag that comprises identifying information regarding the binding agent, and wherein the coding tag is configured to allow transfer of the identifying information regarding the binding agent to the bait nucleic acid or capture nucleic acid attached to the polypeptide analyte immobilized on the solid support.