Back to results
US 11,634,707 B2Granted
Methods and compositions for analyzing cellular components
AssigneeIllumina, Inc.
InventorsKevin L Gunderson, Frank J Steemers, Jeffrey S Fisher, Roberto Rigatti
Life of this patent
Estimating the term…
Abstract
Embodiments of the present invention relate to analyzing components of a cell. In some embodiments, the present invention relate to analyzing components of a single cell. In some embodiments, the methods and compositions relate to sequencing nucleic acids. In some embodiments, the methods and compositions relate to identifying and/or quantitating nucleic acid, proteins, organelles, and/or cellular metabolites.
Claims23
A method of analyzing at least two or more analytes of a plurality of single cells, the method comprising:
- (a) providing a plurality of contiguity preserving elements (CE), wherein each CE comprises a single cell;
- (b) lysing the single cells within the individual CE, wherein the analytes within the single cell are released within the individual CE;
- (c) providing a first reporter moiety to a first analyte within the single cell of each CE;
- (d) providing a second reporter moiety to a second analyte within the single cell of each CE, wherein the first analyte and the second analyte are different types of analytes;
- (e) modifying the analytes such that at least some of the first and second analytes of the individual CE comprise the first and second reporter moieties, respectively;
- (f) combining the individual CE comprising the analytes comprising the reporter moieties;
- (g) compartmentalizing the individual CE comprising the first and second analytes comprising the first and second reporter moieties, respectively into a plurality of compartments, wherein compartments comprise multiple individual CE;
- (h) providing a third reporter moiety to the first analyte comprising the first reporter moiety of each CE, wherein the third reporter moiety provided to the first analyte of each compartment is different from the third reporter moiety provided to the first analyte of each of the other compartments;
- (i) providing a fourth reporter moiety to the second analyte comprising the second reporter moiety of each CE, wherein the fourth reporter moiety provided to the second analyte of each compartment is different from the fourth reporter moiety provided to the second analyte of each of the other compartments;
- (j) further modifying the analytes such that at least some first analytes comprise the first and third reporter moieties and at least some second analytes comprise the second and fourth reporter moieties;
- (k) analyzing said analytes comprising the reporter moieties of each compartment, wherein such analysis detects the single cell which is the source of each analyte.
8 more elements
A method of analyzing at least two or more analytes of a plurality of single cells, the method comprising:
- (a) providing a plurality of contiguity preserving elements (CE), wherein each CE comprises cells;
- (b) providing a first reporter moiety to a first analyte within the cells of each CE;
- (c) providing a second reporter moiety to a second analyte within the cells of each CE, wherein the first analyte and the second analyte are different types of analytes;
- (d) modifying the analytes such that at least some of the first and second analytes of the individual CE comprise the first and second reporter moieties, respectively;
- (e) combining the individual CE comprising the analytes comprising the reporter moieties;
- (f) compartmentalizing the individual CE comprising the first and second analytes comprising the first and second reporter moieties, respectively into a plurality of compartments, wherein compartments comprise multiple individual CE;
- (g) providing a third reporter moiety to the first analyte comprising the first reporter moiety of each CE, wherein the third reporter moiety provided to the first analyte of each compartment is different from the third reporter moiety provided to the first analyte of each of the other compartments;
- (h) providing a fourth reporter moiety to the second analyte comprising the second reporter moiety of each CE, wherein the fourth reporter moiety provided to the second analyte of each compartment is different from the fourth reporter moiety provided to the second analyte of each of the other compartments;
- (i) further modifying the analytes such that at least some first analytes comprise the first and third reporter moieties and at least some second analytes comprise the second and fourth reporter moieties;
- (j) analyzing said analytes comprising the reporter moieties of each compartment, wherein such analysis detects the cell which is the source of each analyte.
7 more elements
Dependencies are inferred from the claim text.