Metabolic pathways with increased carbon yield
AssigneeTotal Raffinage Chimie
InventorAlexei Godina
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Abstract
The present invention relates to the conversion of a carbon source into acetyl phosphate with increased carbon yield. In particular, the invention provides metabolically engineered micro-organisms capable of producing acetyl phosphate from a carbon source with increased carbon yield, which micro-organisms have been transformed with at least one exogenous nucleic acid encoding a phosphoketolase having sedoheptulose-7-phosphate phosphoketolase activity and which are further genetically modified to have eliminated transketolase activity. The invention also provides methods for the production of chemicals using said micro-organisms.
Claims15
A metabolically engineered micro-organism capable of increased acetyl phosphate conversion from a carbon source compared to a corresponding non-metabolically engineered micro-organism, wherein the micro-organism comprises at least one exogenous nucleic acid encoding a phosphoketolase having sedoheptulose-7-phosphate phosphoketolase activity, wherein the phosphoketolase having sedoheptulose-7-phosphate phosphoketolase activity is a Bifidobacterium longum phosphoketolase comprising the sequence set forth in SEQ ID NO:
- 2, and wherein the micro-organism is further genetically modified to have eliminated transketolase activity.
Dependencies are inferred from the claim text.