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US 11,634,698 B2Granted
Nucleases and methods for making and using them
AssigneeBASF SE
InventorsXuqiu Tan, Kenneth Barrett, Jared Dennis, Dongmei Ren, Corey Dodge, Bin Zhang
Life of this patent
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Abstract
Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.
Claims45
A synthetic or recombinant polypeptide comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO:
- 1, wherein the polypeptide has nuclease activity, and wherein the polypeptide comprises one or more mutations of E230L, L114F, E260R, E230M, D246P, S161 R, N54S, T227H, E230R, G263A, G119N, V226K, N127S, P84V, D83E, D28G, V45T, M262V, A190K, P84N, 144R, G256S, A73M, P179L, Q135E, A60P, G263K, S161E, P72N, P84L, S74N, T82R, G75R, Q141R, and D107N.
Dependencies are inferred from the claim text.