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US 11,634,696 B2Granted
Mutant polyhydroxyalkanoate synthetase, gene and transformant thereof, and method for producing polyhydroxyalkanoate
AssigneeKaneka Corporation
InventorsShingo Kobayashi, Shunsuke Sato, Naoaki Taoka
Life of this patent
Estimating the term…
Abstract
Provided is a mutant polyhydroxyalkanoate synthetase having an amino acid sequence which has 85% or more sequence identity with the amino acid sequence of SEQ ID NO: 1 and which contains a mutation of substitution of at least one of the amino acids at the 27th to 33rd, 39th, 56th, 106th, 129th, 144th, 165th, 170th and 172nd to 187th positions from the N-terminus of the amino acid sequence of SEQ ID NO: 1 with another amino acid.
Claims30
A mutant polyhydroxyalkanoate synthetase having at least 95% sequence identity with the amino acid sequence of SEQ ID NO:
- 1 and having at least one mutation selected from the group consisting of (A), (B), (C), and (n):
- mutation (A):
- a substitution of at least one of amino acids at 27th to 33rd positions from the N-terminus of the amino acid sequence of SEQ ID NO:
- 1 with another amino acid;
- mutation (B):
- a substitution of at least one of amino acids at 39th, 56th, 106th, 129th, 144th, 165th and 170th positions from the N-terminus of the amino acid sequence of SEQ ID NO:
- 1 with another amino acid;
- mutation (C):
- a substitution of at least one of amino acids at 172nd to 187th positions except 179th position from the N-terminus of the amino acid sequence of SEQ ID NO:
- 1 with another amino acid, and
- mutation (n):
- a substitution of leucine at the 179th position from the N-terminus of the amino acid sequence of SEQ ID NO:
- 1 with arginine.
10 more elements
Dependencies are inferred from the claim text.