Aldehyde dehydrogenase variants and methods of using same
AssigneeGenomatica, Inc.
InventorsAmit Shah, Joseph Warner
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Abstract
The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.
Claims26
An isolated nucleic acid molecule selected from:
- (a) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence that is a variant of SEQ ID NO:
- 1, wherein said amino acid sequence comprises the amino acid substitution I66M, wherein the amino acid sequence has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 1 and wherein said polypeptide has aldehyde dehydrogenase activity and
- (b) a nucleic acid molecule that is complementary to (a).
An isolated polypeptide comprising an amino acid sequence that is a variant of SEQ ID NO:
- 1, wherein said amino acid sequence comprises the amino acid substitution I66M, wherein the amino acid sequence has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:1 and wherein the isolated polypeptide has aldehyde dehydrogenase activity.
Dependencies are inferred from the claim text.