Human otic progenitor identification and isolation
AssigneeThe University of Sheffield
InventorsCarlos Marcelo Nicolas Rivolta, Sarah Louise Boddy
Life of this patent
Estimating the term…
Abstract
The present invention relates generally to the identification and isolation of human otic progenitor cells. More specifically, the present invention relates to a method of using cell markers to identify and isolate human otic progenitor cells from a mixed population of cells, methods of enrichment and production of human otic progenitor cells, and associated kits for use in identification and/or isolation of human otic progenitor cells, wherein the cell markers are selected from SSEA1 (CD15), disialoganglioside GD3, TRA-2-49 (liver/bone/kidney alkaline phosphatase), SSEA4, ganglioside GD2 and CD141.
Claims15
A method of identifying human otic progenitor cells in a mixed population of cells comprising determining if a cell has at least three cell surface markers selected from SSEA1, GD3, TRA-2-49, SSEA4, GD2 and CD141, wherein the cell markers SSEA1, GD3, TRA-2-49, SSEA4, and GD2 are positive cell markers, and wherein the cell marker CD141 is a negative cell marker, wherein at least two positive cell markers are used in combination with the negative cell marker CD141.
Dependencies are inferred from the claim text.