Production of brewer's wort having increase fermentable sugars for fermentation
AssigneeDupont Nutrition Biosciences Aps
InventorsJacob Flyvholm Cramer, Tove Bladt
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods, compositions, apparatuses and kits comprising two or more distant related glucoamylases in mashing having a synergistic effect on the released fermentable DPI sugar. Increased levels of DPI being primarily glucose is of high importance for high attenuation brewing applied for brewing low carb or light beers. In some embodiments, the present disclosure provides methods, apparatuses; compositions and kits for the use of two or more glucoamylases to increase the apparent specific activity in the mashing operation.
Claims21
A method for producing a brewer's wort having increased DPI sugar comprising adding to a mash comprising a grist a first glucoamylase comprising a Fusarium venenatum glycoamylase as set forth in SEQ ID NO:
- 1 (FvGA) or a sequence having at least 80% identity thereto, a second glucoamylase comprising an Aspergillus fumigatus glucoamylase as set forth in SEQ ID NO:
- 2 (AfuGA) or a sequence having at least 80% identity thereto and a third glucoamylase comprising a Trichoderma reesei variant glucoamylase as set forth in SEQ ID NO:
- 4 (TrGA varl) or a sequence having at least 80% identity thereto to produce the brewer's wort.
Dependencies are inferred from the claim text.