US 11,634,481 B2 · B-cell epitope of Trichinella spiralis cysteine protease inhibitor, hybridoma cell line, monoclonal antibody and uses thereof

Abstract

The present disclosure relates to the field of immunology, in particular to a B-cell epitope of Trichinella spiralis cysteine protease inhibitor, a hybridoma cell line, a monoclonal antibody and uses thereof. The present disclosure provides a hybridoma cell line that can generate anti-WN10 antibody, and identifies the specific B-cell epitope of WN10 protein recognized by the monoclonal antibody. These are of great significance for the diagnosis of trichinellosis, for the establishment of competitive ELISA for detecting antibodies and sandwich ELSIA for detecting circulating antigens, for the detection of Trichinella spiralis in different hosts and for the development of subunit vaccines.

Claims6

Dependent claims

An antibody or antigen-binding fragment thereof capable of specifically binding to Trichinella spiralis WN10 protein, comprising

a CDR-L1, a CDR-L2, and a CDR-L3 in a light chain variable region, and
a CHR-H1, a CDR-H2, and a CDR-H3 in a heavy chain variable region,
wherein the CDR-L1, CDR-L2 and CDR-L3 have an amino acid sequence set forth in SEQ ID NOs:
17, 18 and 19, respectively, and
wherein the CDR-H1, CDR-H2 and CDR-H3 have an amino acid sequence set forth in SEQ ID NOs:
20, 21 and 22, respectively.

3 more elements

Dependencies are inferred from the claim text.

B-cell epitope of Trichinella spiralis cysteine protease inhibitor, hybridoma cell line, monoclonal antibody and uses thereof

Life of this patent

B-cell epitope of Trichinella spiralis cysteine protease inhibitor, hybridoma cell line, monoclonal antibody and uses thereof

Life of this patent

Abstract

Claims6