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US 11,634,476 B2Granted
Mammalian cell culture
AssigneeAmgen Inc.
InventorsBrian D. Follstad, Rebecca E. McCoy, Arvia E. Morris
Life of this patent
Estimating the term…
Abstract
The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures.
Claims41
A method of limiting a mammalian cell culture at a packed cell volume (PCV) of 35% or less, wherein said mammalian cell expresses a recombinant protein, comprising:
- establishing a mammalian cell culture in a serum-free culture medium in a bioreactor by inoculating the bioreactor with at least 0.5×106 to 3.0×106 cells/mL,
- limiting the PCV of said culture by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less,
- maintaining the mammalian cells by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less, and
- monitoring the cell culture, such that the PCV of the culture is maintained at 35% or less.
1 more element
A method of limiting a mammalian cell culture at a viable cell density (VCD) of 1×108 cells/mL or less, wherein said mammalian cell expresses a recombinant protein, comprising:
- establishing a mammalian cell culture in a serum-free culture medium in a bioreactor by inoculating the bioreactor with at least 0.5×106 to 3.0×106 cells/mL,
- limiting the VCD of said culture by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less,
- maintaining the mammalian cells by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less, and
- monitoring the cell culture, such that the VCD of the culture is maintained at 1×108 cells/mL or less.
1 more element
Dependencies are inferred from the claim text.