Method and device for fast dissolution of solid protein composition

A device suitable for dissolving a solid composition in an aqueous solvent, the device comprising:

• a first closed container holding a first solid composition comprising low-density fibrinogen in the form of a porous sponge, the first closed container including a first closed container inlet having a closed state and an open state, wherein in the closed state the first closed container inlet is sealed to the passage of fluid and in the open state the first closed container inlet provides a path for fluid communication into the first closed container;
• a first movable seal element arranged within the first closed container, for sealing the first closed container and configured to maintain sealing of the first closed container while moving therein between at least a first and a second position within the first closed container, to decrease the internal volume of the first closed container;
• the first movable seal element is configured to be releasably held in a first position within the first closed container;
• a first controller for controlling changing of the first closed container inlet between a closed state and an open state,
• a first reservoir suitable for holding a first aqueous solvent, the first reservoir comprising a first reservoir outlet adapted to be in fluid communication with the first closed container inlet;
• and
• a second moveable seal element arranged within the first reservoir configured to maintain sealing of the first reservoir,
• wherein:
• (i) when the first closed container inlet is in a closed state while the first movable seal element is in the second position, and subsequently the first movable seal element is moved to the first position and is kept held, a headspace having a sub-atmospheric pressure is formed, or
• (ii) the device is provided with the closed container further containing a headspace with a sub-atmospheric pressure;
• a closed container inlet;
• and the first movable seal element being held in the first position;
• wherein the first movable seal element is configured to be fixed in the first position while the aqueous solvent is allowed to flow into the interior volume of the closed container, thereby maintaining the headspace at a sub-atmospheric pressure;
• and wherein the first closed container and the first reservoir are configured to allow agitation by repeated transfer of the aqueous solvent between the first reservoir and the first closed container;
• the device being configured such that:
• when the container inlet is in the open state, the aqueous solvent is allowed to flow into the container;
• the container inlet is configured to be in the closed state after the aqueous solvent has flowed within the container;
• and when the container inlet is in the closed state, the first movable seal element is further configured to be released and to move from the first to the second position within the first closed container by a difference between atmospheric pressure outside the closed container and the sub-atmospheric pressure in the headspace, thereby decreasing the internal volume of the first closed container, such that the internal pressure within the first closed container is increased without allowing entry of gas into the container.