Cell sorter in a sealed system under controlled atmosphere

A sealed chamber comprising:

• a processing chamber sealed from the outside air including an extended glove front coupled to and extending from a front face of the processing chamber, the extended glove front comprising a door coupled to the front face and a pair of gloves extending inwardly into the processing chamber;
• a buffer module adjacent to the processing chamber and having a side door to insert a test subject, an inner door to access the processing chamber, and an airlock system capable of modifying the air content within the buffer module;
• and
• an enclosed holder in the sealed chamber that is capable of holding the test subject and having a removable cap and a plunger configured to sequester the test subject in a part of the holder;
• wherein the sealed chamber is a sealed system such that cells can be placed under consistent conditions such that cells can be sorted, obtained, and analyzed under consistent conditions.