Method for producing cell tissue, and porous film
AssigneeFUJIFILM Corporation
InventorsKoju Ito, Shun Goto, Souichi Kohashi, Kohji Nakazawa, Hiroshi Yabu
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Abstract
Provided are a method for producing a cell tissue, including a culturing step of culturing cells capable of serving as a feeder inside opening pores and communicating pores of a porous film having a plurality of the opening pores provided on a surface thereof and the communicating pores communicating mutually adjacent opening pores with one another; and a porous film including a plurality of opening pores provided on a surface thereof and communicating pores communicating mutually adjacent opening pores with one another.
Claims13
A method for producing a cell tissue, comprising:
- a culturing step of co-culturing cells capable of serving as a feeder and at least one of vascular endothelial cells or lymphatic endothelial cells inside opening pores and communicating pores of a porous film having a plurality of the opening pores provided on a surface thereof and the communicating pores communicating mutually adjacent opening pores with one another, wherein
- the porous film is a monolayer of a porous layer comprising the opening pores and the communicating pores, or a laminate consisting of a monolayer of a porous layer disposed on a substrate, the porous layer comprising the opening pores and the communicating pores,
- an opening diameter of the opening pore is in a range of 18 μm to 44 μm,
- a depth of the opening pore is in a range of 18 μm to 48 μm,
- a pore diameter of the communicating pore is in a range of 14 μm to 40 μm, and
- the plurality of opening pores, which have an isotropic shape and the same size in the plane direction, are arranged in a honeycomb shape on the surface of the porous film.
A porous film comprising:
- a plurality of opening pores provided on a surface thereof;
- and
- communicating pores communicating mutually adjacent opening pores with one another, wherein
- the porous film is a monolayer of a porous layer comprising the opening pores and the communicating pores, or a laminate consisting of a monolayer of a porous layer disposed on a substrate, the porous layer comprising the opening pores and the communicating pores,
- an opening diameter of the opening pore is in a range of 18 μm to 44 μm,
- a depth of the opening pore is in a range of 18 μm to 48 μm,
- a pore diameter of the communicating pore is in a range of 14 μm to 40 μm,
- a major axis of the opening pore in a plane direction of the porous film is in a range of 20 μm to 100 μm, and
- the plurality of opening pores, which have an isotropic shape and the same size in the plane direction, are arranged in a honeycomb shape on the surface of the porous film.
A porous film comprising:
- a plurality of opening pores provided on a surface thereof;
- and communicating pores communicating mutually adjacent opening pores with one another, wherein the porous film is a monolayer of a porous layer comprising the opening pores and the communicating pores, or a laminate consisting of a monolayer of a porous layer disposed on a substrate, the porous layer comprising the opening pores and the communicating pores, an opening diameter of the opening pore is in a range of 18 μm to 44 μm, a depth of the opening pore is in a range of 18 μm to 48 μm, a pore diameter of the communicating pore is in a range of 14 μm to 40 μm, a depth of the opening pore is in a range of 20 μm to 100 μm, and the plurality of opening pores, which have an isotropic shape and the same size in the plane direction, are arranged in a honeycomb shape on the surface of the porous film.
Dependencies are inferred from the claim text.