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AssigneeNajit Technologies, Inc.
InventorsIan J. Amanna, Elizabeth A. Poore
Estimating the term…
Provided are surprisingly effective methods for inactivating pathogens, and for producing highly immunogenic vaccine compositions containing an inactivated pathogen rendered noninfectious by exposure to a Fenton reagent, or by exposure to a Fenton reagent or a component thereof in combination with a methisazone reagent selected from the group consisting of methisazone, methisazone analogs, functional group(s)/substructure(s) of methisazone, and combinations thereof. The methods efficiently inactivate pathogens, while substantially retaining pathogen antigenicity and/or immunogenicity, and are suitable for inactivating pathogens, or for the preparation of vaccines for a wide variety of pathogens with genomes comprising RNA or DNA, including viruses and bacteria. Also provided are highly immunogenic inactivated vaccine compositions prepared by using any of the disclosed methods, and methods for eliciting an immune response in a subject by administering such vaccine compositions.
A method for inactivating a pathogen, the method comprising contacting a pathogen having an RNA or DNA genome with hydrogen peroxide, or with a Fenton reagent containing hydrogen peroxide in combination with at least one transition metal selected from the group consisting of Cu and Fe, and in either case with a methisazone reagent, in an amount and for a time-period sufficient to render the pathogen noninfectious, wherein inactivation of the pathogen proceeds at an increased rate relative to that produced by contacting the pathogen with either the hydrogen peroxide or the Fenton reagent alone.
Dependencies are inferred from the claim text.