Enzymes and methods for cleaving n-glycans from glycoproteins
AssigneeThe Regents of the University of California
InventorsDaniel Garrido, J. Bruce German, Carlito B. Lebrilla, David A. Mills
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Abstract
Provided herein are deglycosylating enzymes that remove a broad range of N-glycans from N-glycosylated proteins. Further provided are methods of recombinantly producing and expressing the deglycosylating enzymes. The presently described deglycosylating enzymes can be used to produce free glycans for characterization, and for prebiotic and immunostimulatory uses. In addition, the presently described deglycosylating enzymes can be used to produce deglycosylated proteins for characterization, to improve digestion, and to reduce immunogenicity.
Claims21
A composition comprising:
- (i) a recombinant polypeptide comprising a sequence at least 80% identical to SEQ ID NO:4 or SEQ ID NO:5 wherein said polypeptide can cleave high mannose, complex, and hybrid N-glycans from a glycoprotein and wherein the polypeptide lacks a transmembrane domain;
- and
- (ii) a glycoprotein, wherein the glycoprotein comprises a high mannose, complex, or hybrid N-glycan.
A composition comprising a recombinant cell expressing a recombinant polypeptide comprising a sequence at least 80% identical to SEQ ID NO:4 or SEQ ID NO:5, wherein said polypeptide can cleave high mannose, complex, and hybrid N-glycans from a glycoprotein and wherein the polypeptide lacks a transmembrane domain.
Dependencies are inferred from the claim text.