Specimen imaging with x-ray and optical measurement

A system for specimen imaging with combined x-ray and optical measurement comprising:

• a micro-X-ray computed tomography (CT) unit configured to provide voxel-based CT images of the specimen;
• a structured light imaging (SLI) unit configured to provide optical images obtained at a plurality of wavelengths, a plurality of structured light phases, and at a plurality of structured-light pattern periods;
• an image processing unit configured to receive the CT images and the optical images and configured by firmware in memory to:
• demodulate the structured light images;
• register the CT and optical images;
• extract like-tissue volumes from the CT images to form a volume map;
• process the optical images to determine texture at a plurality of subimages of the optical images, the determined textures forming a texture map;
• use a first machine-learning-based classifier to determine a surface tissue type map of the specimen from the texture map;
• and
• use a second machine-learning-based classifier to determine a three-dimensional tissue-type map from the surface tissue type map and the extracted like-tissue volumes from the CT images.