Mantle phenotype detection in palm
AssigneeMalaysian Palm Oil Board
InventorsMeilina Ong Abdullah, Ooi Siew Eng, Leslie Low Eng Ti, Rajinder Singh, Rajanaidu Nookiah, Ravigadevi Sambanthamurthi, Nan Jiang, Steven W. Smith, Nathan D. Lakey, Rob Martienssen, Jared Ordway, Michael Hogan
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Abstract
Methods, compositions, kits, and computer program code are provided for predicting somaclonal abnormality (e.g., a Mantled phenotype) in a plant and or sorting plants based on the predicted presence or absence of somaclonal abnormality.
Claims10
A method for physically separating the oil palm plant or seed predicted to have the Mantled phenotype from one or more oil palm plants or seeds predicted to lack the Mantled phenotype based on the methylation status, the method comprising:
- obtaining genotype information for a sample from the oil palm seeds or plants, wherein the genotype information comprises methylation status of at least one cytosine within a differential methylation region (DMR), wherein the DMR is within a sequence of DNA at least 95% identical to SEQ ID NO:66 and predicting, based on the obtained genotype information, presence or absence of the Mantled phenotype of the palm plants or seeds, or
- obtaining predicted presence or absence of the Mantled phenotype of palm plants or seeds based on methylation status of at least one cytosine within the DMR;
- and,
- physically separating the oil palm plant or seed predicted to have the Mantled phenotype from one or more oil palm plants or seeds predicted to lack the Mantled phenotype based on the methylation status, wherein a reduced methylation status of at least one cytosine within the DMR relative to a control locus indicates presence of the Mantled phenotype.
Dependencies are inferred from the claim text.